The Basic Principles Of high performance liquid chromatography
To forestall the lack of stationary stage, which shortens the column’s life time, it can be sure covalently into the silica particles. Bonded stationary phases. HPLC separation of a mixture of flavonoids with UV/Vis detection at 360 nm and, inside the inset, at 260 nm. The choice of wavelength has an effect on Just about every analyte’s signal.